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- Organic Geochemistry
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- Hinrichs Lab - Deep Life Community
Hinrichs Lab - Deep Life Community
Experimental calibration of methane clumped isotopologue systematics for microbial production and consumption
| Duration: | May 2014 - October 2014 |
| Funding: | Deep Carbon Observatory (DCO), Deep Life |
| Principal Investigator(s): | Martin Könneke |
| Involved scientists in the Hinrichs Lab: | |
| International partner(s): | Shuhei Ono (EAPS, Massachusetts Institute of Technology, Cambridge, USA) |
Abstract
This project will acquire crucial experimental data on the effects of various methanogenic substrates as well as the anaerobic methane oxidation on the clumped isotopologue systematics of methane.
We want to study the abundance of doubly-isotope substituted methane (13CH3D) during methanogenesis and anaerobic methane oxidation. Pure culture studies with Methanosarcina species will allow the formation of methane under highly defined and reproducible conditions in order to correlate the abundance of doubly substituted methane isotopology with temperature, growth phase and substrates converted to methane. Furthermore, we aim to test the influence of various ANME enrichtments on the clumped isotope composition during microbial methane consumption.
This project will acquire crucial experimental data on the effects of various methanogenic substrates as well as the anaerobic methane oxidation on the clumped isotopologue systematics of methane.
We want to study the abundance of doubly-isotope substituted methane (13CH3D) during methanogenesis and anaerobic methane oxidation. Pure culture studies with Methanosarcina species will allow the formation of methane under highly defined and reproducible conditions in order to correlate the abundance of doubly substituted methane isotopology with temperature, growth phase and substrates converted to methane. Furthermore, we aim to test the influence of various ANME enrichtments on the clumped isotope composition during microbial methane consumption.