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Microbiology
The microbiology working area will be tailored according to the scientific objectives of an expedition and is dedicated to initial handling of samples for microbiological analyses like:
- subsampling
- conservation / fixing / staining of subsampling
- initial analyses, e.g. activity measurements
Glove box
There is a Coy vinyl glove box available on request. This is a one person medium size model. The glove bag is 111 x 71 x 66 cm (L x H x D). The main aperture is 50.8 cm in diameter. There is an air lock for taking samples in and out the glove box chamber. The doors of the airlock chamber are 23 x 23 cm.
Any additional accessories that are essential for a specific project (e.g. oxygen scrubbers, vacuum system) need to be requested at least 3 months ahead the start of an expedition.
Any additional accessories that are essential for a specific project (e.g. oxygen scrubbers, vacuum system) need to be requested at least 3 months ahead the start of an expedition.
Anti-static Ionizer:
PLAS-LABS® Work station anti-static ionizer 800-AS/SPI (AC input 220-240 V). Non-air assisted anti-staic unit to neutralize static within glove boxes.
- Size: WxHxD 4.25”x5.0”x2.5”
- Pulse frequency: Adjustable 1-5 pulse/sec
- Ion coverage: up to 36” / 91 cm
- No airflow required for optimum efficiency
PLAS-LABS® Work station anti-static ionizer 800-AS/SPI (AC input 220-240 V). Non-air assisted anti-staic unit to neutralize static within glove boxes.
- Size: WxHxD 4.25”x5.0”x2.5”
- Pulse frequency: Adjustable 1-5 pulse/sec
- Ion coverage: up to 36” / 91 cm
- No airflow required for optimum efficiency
Furnace
Nabertherm Laboratory Furnace LP 9/12
Technical Details:
Max. temp.: 1200°C
Capacity: 9L
Size (interior): width x depth x height: 230 x 240 x 170 mm
Technical Details:
Max. temp.: 1200°C
Capacity: 9L
Size (interior): width x depth x height: 230 x 240 x 170 mm
Hybridization oven
Hybridisation oven
Major Science® Hybridisation oven with touch screen and rotisserie function.
- Model: MO-ARK
- Speed: 5 – 100 rpm (1 rpm steps)
- Timer: 1 – 9999 min
- Shaker motion/speed: Rocking / 5 – 100rpm
- Temperature: ambient 5 - 85°C (±0.2°C)
- Inner size: 340 x 225 x 260mm (W x L x H)
- Overall dimension 442 x 462 x 452mm (W x L x H)
- Power supply: 220V / ~ 50/60Hz
Major Science® Hybridisation oven with touch screen and rotisserie function.
- Model: MO-ARK
- Speed: 5 – 100 rpm (1 rpm steps)
- Timer: 1 – 9999 min
- Shaker motion/speed: Rocking / 5 – 100rpm
- Temperature: ambient 5 - 85°C (±0.2°C)
- Inner size: 340 x 225 x 260mm (W x L x H)
- Overall dimension 442 x 462 x 452mm (W x L x H)
- Power supply: 220V / ~ 50/60Hz
Incubator
Memmert Incubator IN 55
Technical Details:
Temperature range: 5 - 80°C
Capacity: 53 L
Size (interior): width x depth x height: 400 x 400 x 330 mm
Technical Details:
Temperature range: 5 - 80°C
Capacity: 53 L
Size (interior): width x depth x height: 400 x 400 x 330 mm
Centrifuge
Eppendorf-Centrifuge 5430 R
Technical Details:
Max. rcf: 30,130 x g
Max. rpm: 17,500 rpm
Max. capacity: 30 x 1.5 / 2.0 ml or 6 x 50 ml
Temp.-range: 4°C to 40°C
Technical Details:
Max. rcf: 30,130 x g
Max. rpm: 17,500 rpm
Max. capacity: 30 x 1.5 / 2.0 ml or 6 x 50 ml
Temp.-range: 4°C to 40°C
- Rotor FA-45-30-11 for 30x 1,5/2,0 ml Eppendorf reactionsvials (left)
- Fixed-angle rotor F-35-6-30 incl. rotorlid and adapter for 15/50 ml Falcon tubes (right)
Fluorescence Mikroscope
The Olympus BX-53 PHAKO is a stereo microscope suitable for fluorescence microscopy equipped with a Olympus DP-21digital camera.
| Observation tube | WHN 10x/1-2 |
| Objectives | 10x, 40x, 100x |
| Illumination | transmission light, Hg bulb |
| Camera | Olympus DP-21 |
Different filter sets can be used. At the moment the microscope is equipped with these filters:
| Filter sets | Excitation Filter Wavelength (nm) | Dichromatic Mirror Cut-on Wavelength (nm) | Barrier Filter Wavelength (nm) |
| 1 (Olympus U-FBW) | 460-495 | 505 | 510 |
| 2 (F46-001 CFP ET) | 436 | 455 | 480 |
Vortex Shaker
Vortex mixer
Vortex-Genie® 2 laboratory shaker by Scientific Industries Inc® with several different tube foam inserts.
- Model: G560E
- Size: D x W x H 6.5“ x 4.8“ x 6.5“
- Power supply: 230V - European Plug
- Speed: 600-3200 rpm
Vortex-Genie® 2 laboratory shaker by Scientific Industries Inc® with several different tube foam inserts.
- Model: G560E
- Size: D x W x H 6.5“ x 4.8“ x 6.5“
- Power supply: 230V - European Plug
- Speed: 600-3200 rpm
VWR ECN 44-1372 Test Tube Shaker
Technical Details:
Motion: circular and vibrating
Frequency: 0-2400RPM
Orbit: 5 mm
Tube diameter: max. 20mm
Weight: 2.8 kg
Dimensions: WxHxD 134 x 105 x 172 mm
Voltage: 230V/50Hz
Protection: IP 22
Operation temp: 5 – 40°C
Motion: circular and vibrating
Frequency: 0-2400RPM
Orbit: 5 mm
Tube diameter: max. 20mm
Weight: 2.8 kg
Dimensions: WxHxD 134 x 105 x 172 mm
Voltage: 230V/50Hz
Protection: IP 22
Operation temp: 5 – 40°C
CertoClave
CertoClav EL sterilizer with wire basket (125/140°C)
Technical Details:
Temperature/pressure ranges: 125°C (1,4 bar / 20 psi)/140°C (2,7 bar / 39 psi)
Application areas: Sterilisation, culture medium preparation
Sterilisation goods: Solid instruments, liquids (culture mediums), bottles and equipment
ø (inside): 24 cm
Inside height: 39 cm
Diagonal: 46,5 cm
Weight: 9 kg
Material: Aluminium
Heating: Integrated
Rated voltage: 230 V AC, 50-60 Hz
Performance: 1900 Watt
Temperature indicator: Precision thermometer
Pressure indicator: Precision manometer
Safety: 4-fold pressure relief protection device
Technical Details:
Temperature/pressure ranges: 125°C (1,4 bar / 20 psi)/140°C (2,7 bar / 39 psi)
Application areas: Sterilisation, culture medium preparation
Sterilisation goods: Solid instruments, liquids (culture mediums), bottles and equipment
ø (inside): 24 cm
Inside height: 39 cm
Diagonal: 46,5 cm
Weight: 9 kg
Material: Aluminium
Heating: Integrated
Rated voltage: 230 V AC, 50-60 Hz
Performance: 1900 Watt
Temperature indicator: Precision thermometer
Pressure indicator: Precision manometer
Safety: 4-fold pressure relief protection device
Ultrasonic Bath
Bandelin Sonorex Super RK 106
Technical Details:
Diameter: 240 mm
Height: 130 mm
Capacity: 5 L
Time switch: 1 - 15 min. + permanent
Technical Details:
Diameter: 240 mm
Height: 130 mm
Capacity: 5 L
Time switch: 1 - 15 min. + permanent
Pure water
Ultra pure water (Type I, 18.2 MΩ-cm) suitable for geochemical and microbiological lab purposes is produced from pre-purified water (by an upstream SERADEST ion exchanger cartridge) with a PURELAB Classic device (ELGA).
Activity measurements
One of the most sensitive tests to detect surface or water attached microbes, i.e., living cells, is the ATP test with a luminometer and the firefly-based enzyme assay. The surfaces can be sampled with a specific type of Q-tips, and the numerical results are available within seconds.
When ATP is brought into contact with the reagent combination luciferin/luciferase, a reaction takes place which emits light in direct proportion to the amount of ATP present. The device measures the amount of light generated and provides information as to how much biological activity is present.
When ATP is brought into contact with the reagent combination luciferin/luciferase, a reaction takes place which emits light in direct proportion to the amount of ATP present. The device measures the amount of light generated and provides information as to how much biological activity is present.
Pumps
Portable sampling pump drive
Portable sampler (pump drive) MASTERFLEX® E/S® by Cole-Parmer® with rechargeable battery and protective housing.
- Size: H x W x D 16" x 11-1/3" x 10-1/2"
- Pump speed: 70 – 400 rpm, with reversible flow and prime/purge function
- Housing: Floatable (up to 30 min), control panel splash proof
- Power supply: 12 VDC rechargeable battery (up to 4 hrs) / AC or 12 VDC power supply
- For Masterflex® L/S® standard, Quick Load®, PTFE tubing and Easy-Load® pump heads.
Portable sampler (pump drive) MASTERFLEX® E/S® by Cole-Parmer® with rechargeable battery and protective housing.
- Size: H x W x D 16" x 11-1/3" x 10-1/2"
- Pump speed: 70 – 400 rpm, with reversible flow and prime/purge function
- Housing: Floatable (up to 30 min), control panel splash proof
- Power supply: 12 VDC rechargeable battery (up to 4 hrs) / AC or 12 VDC power supply
- For Masterflex® L/S® standard, Quick Load®, PTFE tubing and Easy-Load® pump heads.
Tubing pump:
ISMATEC® High precision multichannel pump with planetary drive for low-flow, multi-channel fluid transfer.
- Model: IPC with 4 channels (ISM 930)
- Flow rate: 0.002 – 44 ml/min (per channel)
- Speed setting: in %, resolution 0.1% (rpm) / in µl/min or ml/min (flow rate)
ISMATEC® High precision multichannel pump with planetary drive for low-flow, multi-channel fluid transfer.
- Model: IPC with 4 channels (ISM 930)
- Flow rate: 0.002 – 44 ml/min (per channel)
- Speed setting: in %, resolution 0.1% (rpm) / in µl/min or ml/min (flow rate)
Vacuum pump
Millipore® chemical duty vacuum/pressure pump WP61 220 50 with chemically-resistant head and diaphragm.
- Size: Lx W x H 10.5”x 9.4”x 9.7”
- Power supply: 230 V, 50 Hz, 1.7 A
- Application: Vacuum source for filter holders, manifolds, and devices.
Millipore® chemical duty vacuum/pressure pump WP61 220 50 with chemically-resistant head and diaphragm.
- Size: Lx W x H 10.5”x 9.4”x 9.7”
- Power supply: 230 V, 50 Hz, 1.7 A
- Application: Vacuum source for filter holders, manifolds, and devices.
Sample storage
A deep freezer (-80°C) with a capacity of 445 l is installed in the Microbiological Lab Container. Additional chest freezers (as low as -80°C) are available on request. Samples can also be stored at +4 °C in core reefer containers.