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Publications

in chronological order:

* To whom correspondence should be addressed

Buck-Wiese, H., Andskog, M.A., Nguyen, N.P., Bligh, M., Asmala, E., Vidal Melgosa, S., Liebeke, M., Gustafsson, C., Hehemann, J.H. (December 2022). Fucoid brown algae inject fucoidan carbon into the ocean. Proc Natl Acad Sci U S A, 120(1), e2210561119. https://doi:10.1073/pnas.2210561119

Beidler, I., Robb, C.S., Vidal Melgosa, S., Zuhlke, M.K., Bartosik, D., Solanki, V., Markert, S., Becher, D., Schweder, T., Hehemann, J.H. (november 2022). Marine bacteroidetes use a conserved enzymatic cascade to digest diatom β-mannan. ISME Journal. https://doi:10.1038/s41396-022-01342-4

Steinke N., Vidal-Melgosa S., Schultz-Johansen M., Hehemann J.-H.  (June 2022) Biocatalytic quantification of α-glucan in marine particulate organic matter. Wiley online library. https://doi.org/10.1002/mbo3.1289

Solanki, V., Krüger, K., Crawford, C.J., Pardo-Vargas, A., Danglad-Flores, J., Le Mai Hoang, K., Klassen, L., Abbott, D.W., Seeberger, P.H., Aman, R.I., Teeling, H., Hehemann, J.-H. (April 2022) Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi.ISME J. https://doi.org/10.1038/s41396-022-01223-w

Bäumgen, M., Dutschei, T., Bartosik, D., Suster, C., Reisky, L., Gerlach, N., Stanetty, C., Mihovilovic, M.D., Schweder, T., Hehemann J.-H. and Bornscheuer, U.T. (2021). "A new carbohydrate-active oligosaccharide dehydratase is involved in the degradation of ulvan." Journal of Biological Chemistry,  DOI:https://doi.org/10.1016/j.jbc.2021.101210

Huang, G., Vidal-Melgosa, S., Sichert, A., Becker, S., Fang, Y., Niggemann, J., Iversen, M.H., Cao, Y., Hehemann, J.-H. (September 2021). Secretion of sulfated fucans by diatoms may contribute to marine aggregate formation ASLO Association for the Sciences of Limnology and Oceanography https://doi.org/10.1002/lno.11917

Orellana, L. H., Francis, T.B., Ferrano, M., Hehemann, J.-H., Fuchs, B.M., Amann, R. (September 2021). Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms The ISME Journal https://doi.org/10.1038/s41396-021-01105-7

Dittmar, Th., Lennartz, S., Buck-Wiese, H., Hansell, D., Santinelli, C., Vanni, C., Blasius, B., Hehemann, J.-H. (june 2021). Enigmatic persistence of dissolved organic matter in the ocean Nature Reviews Earth & Environment https://doi.org/10.1038/s43017-021-00183-7

Vidal-Mel­gosa, S., Sich­ert, A., Fran­cis, T.Ben, Bar­tosik, D., Nigge­mann, J., Wichels, A., Wil­lats Wil­liam, G.T., Fuchs, B.M., Teel­ing, H., Becher, D., Schweder, Th., Amann, R., Hehem­ann, J.-H. (Feb­ru­ary 2021). Diatom fucan polysaccharide precipitates carbon during algal blooms. Nature Com­mu­nic­a­tions https://doi.org/10.1038/s41467-021-21009-6

Sichert, A., Le Gall, S., Klau, L.J., Laillet, B., Rogniaux, H., Lillelund Aachmann, F., Hehemann, J.-H. (July 2020). Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae. Glycobiology. https://doi.org/10.1093/glycob/cwaa064

Arnosti, C., Wietz, M., Brinkhoff, T., Hehemann, J.-H., Probandt, D., Zeugner, L., Amann, R. (July 2020). The biogeochemistry of marine polysaccharides: sources, inventories, and bacterial drivers of the carbohydrate cycle Annual Review of Marine Science https://doi.org/10.1146/annurev-marine-032020-012810

Sichert, A., Corzett, Ch.H., Schechter, M.S., Unfried, F., Markert, St., Becher, D., Fernandez-Guerra, A., Liebeke, M., Schweder, Th., Polz, M.F., Hehemann, J.-H. (May 2020). Verrucomicrobia use hundreds of enzymes to digest the algal polysaccharide fucoidan. Nature microbiology, https://doi.org/10.1038/s41564-020-0720-2

Buck-Wiese, H., Fanuel, M., Liebeke, M., Le Mai Hoang, K., Pardo-Vargas, A., Seeberger, P.H., Hehemann, J.-H., Rogniaux, H., Jackson, G.P., Ropartz, D. (April 2020). Discrimination of β-1, 4-and β-1, 3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry Journal of the American Society for Mass Spectrometry https://pubs.acs.org/doi/10.1021/jasms.0c00087

Becker, S., Tebben, J., Coffinet, S., Wilt­shire, K., Iversen, M.H., Harder, T., Hin­richs, K.-U., Hehem­ann, J.-H*. Laminarin is a major molecule in the marine carbon cyclePro­cee­dings of the Na­tio­nal Aca­de­my of Sci­en­ces of the United Sta­tes of Ame­ri­ca (March 2020). https://doi.org/10.1073/pnas.1917001117

Wanke, A., Rovenich, H., Schwanke, F., Velte, S., Becker, S., Hehemann, J., … Zuccaro, A. (2020). Plant species‐specific recognition of long and short β‐1,3‐linked glucans is mediated by different receptor systems. The Plant Journal. https://doi.org/10.1111/tpj.14688

Reisky, L., Préchoux, A., Zühlke, M.-K., Bäumgen, M., Robb, C. S., Gerlach, N., … Schweder, T.*, Bornsscheuer, U.*, Hehemann, J.-H.* (2019). A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan. Nature Chemical Biology. https://doi.org/10.1038/s41589-019-0311-9

Hehemann, J.-H.*, Reintjes, G., Amann, R., Abbott, D. W., Klassen, L., Smith, A. D., … Abbott, D. W.* (2019). Single cell fluorescence imaging of glycan uptake by intestinal bacteria. The ISME Journal. https://doi.org/10.1038/s41396-019-0406-z

Wawra, S., Fesel, P., Widmer, H., Neumann, U., Lahrmann, U., Becker, S., Hehemann, J.-H., Langen, G., Zuccaro, A. (2019). FGB1 and WSC3 are in planta- induced β -glucan-binding fungal lectins with different functions. New Phytologist. https://doi.org/10.1111/nph.15711

Scheschonk, L., Becker, S., Hehemann, J., Diehl, N., Karsten, U., & Bischof, K. (2019). Arctic kelp eco-physiology during the polar night in the face of global warming: a crucial role for laminarin. Marine Ecology Progress Series. https://doi.org/10.3354/meps12860

Robb, C. S., Reisky, L., Bornscheuer, U., & Hehemann, J.-H.* (2018). Specificity and mechanism of carbohydrate demethylation by cytochrome P450 monooxygenases. Biochemical Journal, (2), BCJ20180762. https://doi.org/10.1042/BCJ20180762

Mystkowska, A. A., Robb, C., Vidal-Melgosa, S., Vanni, C., Fernandez-Guerra, A., Höhne, M., & Hehemann, J.-H.* (2018). Molecular recognition of the beta-glucans laminarin and pustulan by a SusD-like glycan binding protein of a marine Bacteroidetes. The FEBS Journal. https://doi.org/10.1111/febs.14674

Chen, J., Robb, C. S., Unfried, F., Kappelmann, L., Markert, S., Song, T., … Hehemann, J.-H., Schweder, T., Teeling, H. (2018). Alpha- and beta-mannan utilization by marine Bacteroidetes. Environmental Microbiology. https://doi.org/10.1111/1462-2920.14414

Koch, H., Dürwald, A., Schweder, T., Noriega-Ortega, B., Vidal-Melgosa, S., Hehemann, J.-H., … Wietz, M.(2018). Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. The ISME Journal. https://doi.org/10.1038/s41396-018-0252-4

Kappelmann, L., Krüger, K., Hehemann, J.-H., Harder, J., Markert, S., Unfried, F., … Teeling, H. (2018). Polysaccharide utilization loci of North Sea Flavobacteriia as basis for using SusC/D-protein expression for predicting major phytoplankton glycans. The ISME Journal. https://doi.org/10.1038/s41396-018-0242-6 

Unfried, F., Becker, S., Robb, C. S., Hehemann, J.-H., Markert, S., Heiden, S. E., … Schweder, T. (2018). Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms. The ISME Journal. https://doi.org/10.1038/s41396-018-0243-5

Reisky, L., Stanetty, C., Mihovilovic, M. D., Schweder, T., Hehemann, J., & Bornscheuer, U. T. (2018). Biochemical characterization of an ulvan lyase from the marine flavobacterium Formosa agariphila KMM 3901T. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-018-9142-y

Hettle, A. G., Vickers, C., Robb, C. S., Liu, F., Withers, S. G., Hehemann, J.-H., & Boraston, A. B. (2018). The Molecular Basis of Polysaccharide Sulfatase Activity and a Nomenclature for Catalytic Subsites in this Class of Enzyme. Structure, 26(5), 747–758.e4. https://doi.org/10.1016/j.str.2018.03.012

Becker, S., & Hehemann, J.-H.* (2018). Laminarin Quantification in Microalgae with Enzymes from Marine Microbes. BIO-PROTOCOL, 8(8), 1–14. https://doi.org/10.21769/BioProtoc.2666

Corzett, C. H., Elsherbini, J., Chien, D. M., Hehemann, J.-H., Henschel, A., Preheim, S. P., … Polz, M. F. (2018). Evolution of a Vegetarian Vibrio : Metabolic Specialization of V. breoganii to Macroalgal Substrates. Journal of Bacteriology, (April), JB.00020-18. https://doi.org/10.1128/JB.00020-18

Reisky, L., Büchsenschütz, H. C., Engel, J., Song, T., Schweder, T., Hehemann, J.-H.*, & Bornscheuer, U. T.* (2018). Oxidative demethylation of algal carbohydrates by cytochrome P450 monooxygenases. Nature Chemical Biology. https://doi.org/10.1038/s41589-018-0005-8

The CAZypedia Consortium. (2017). Ten years of CAZypedia: A living encyclopedia of carbohydrate-active enzymes. Glycobiology. https://doi.org/10.1093/glycob/cwx089

Robb, C. S., Mystkowska, A. A., & Hehemann, J.-H.* (2017). Crystal structure of a marine glycoside hydrolase family 99 related protein lacking catalytic machinery. Protein Science. https://doi.org/10.1002/pro.3291

Badur, A. H., Plutz, M. J., Yalamanchili, G., Jagtap, S. S., Schweder, T., Unfried, F., Markert S., Polz M. F., Hehemann J.-H.*, Rao, C. V.* (2017). Exploiting Fine-scale Genetic and Physiological Variation of Closely Related Microbes to Reveal Unknown Enzyme Functions. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M117.787192

Li, D., Yewalkar, S., Bi, X., Duff, S., Posarac, D., Wang, H., Woodfin, L. A., Hehemann, J.-H., Potter, S. C., Nano, F. E. (2017). Heterologous expression of LamA gene encoded endo-β-1,3-glucanase and CO2 fixation by bioengineered Synechococcus sp. PCC 7002. Frontiers of Environmental Science & Engineering, 11(2), 9. https://doi.org/10.1007/s11783-017-0910-1

Hehemann, J.-H.*, Truong, L. Van, Unfried, F., Welsch, N., Kabisch, J., Heiden, S. E., … Schweder, T.* (2017). Aquatic adaptation of a laterally acquired pectin degradation pathway in marine Gammaproteobacteria. Environmental Microbiology. https://doi.org/10.1111/1462-2920.13726

Becker, S., Scheffel, A., Polz, M. F., & Hehemann, J.-H.* (2017). Accurate quantification of laminarin in marine organic matter with enzymes from marine microbes. Applied and Environmental Microbiology. https://doi.org/10.1128/AEM.03389-16

Hehemann, J.-H., Arevalo, P., Datta, M. S., Yu, X., Corzett, C. H., Henschel, A., … Polz, M. F. (2016). Adaptive radiation by waves of gene transfer leads to fine-scale resource partitioning in marine microbes. Nature Communications, 7, 12860. https://doi.org/10.1038/ncomms12860

Hobbs, J. K., Lee, S. M., Robb, M., Hof, F., Barr, C., Abe, K. T., Hehemann J.-H., McLean R., Abbott D. W., Boraston, A. B. (2016). KdgF, the missing link in the microbial metabolism of uronate sugars from pectin and alginate. Proceedings of the National Academy of Sciences of the United States of America, 113(22), 6188–6193. https://doi.org/10.1073/pnas.1524214113

Labourel, A., Jam, M., Legentil, L., Sylla, B., Hehemann, J. H., Ferrieres, V., … Michel, G. (2015). Structural and biochemical characterization of the laminarinase ZgLamCGH16 from Zobellia galactanivorans suggests preferred recognition of branched laminarin. Acta Crystallographica. Section D, Biological Crystallography, 71(Pt 2), 173–184. https://doi.org/10.1107/S139900471402450X

Hehemann, J.-H.*, Boraston, A. B., & Czjzek, M.* (2014). A sweet new wave: structures and mechanisms of enzymes that digest polysaccharides from marine algae. Current Opinion in Structural Biology, 28, 77–86. https://doi.org/10.1016/j.sbi.2014.07.009

Hehemann, J.-H.*, Law, A., Redecke, L., & Boraston, A. B.* (2014). The structure of RdDddP from Roseobacter denitrificans reveals that DMSP lyases in the DddP-family are metalloenzymes. PloS One, 9(7), e103128. https://doi.org/10.1371/journal.pone.0103128

Jagtap, S. S., Hehemann, J.-H., Polz, M. F., Lee, J.-K., & Zhao, H. (2014). Comparative biochemical characterization of three exolytic oligoalginate lyases from Vibrio splendidus reveals complementary substrate scope, temperature, and pH adaptations. Applied and Environmental Microbiology, 80(14), 4207–4214. https://doi.org/10.1128/AEM.01285-14

Labourel, A., Jam, M., Jeudy, A., Hehemann, J.-H., Czjzek, M., & Michel, G. (2014). The β-glucanase ZgLamA from Zobellia galactanivorans evolved a bent active site adapted for efficient degradation of algal laminarin. The Journal of Biological Chemistry, 289(4), 2027–42. https://doi.org/10.1074/jbc.M113.538843

Pagarete, A., Kusonmano, K., Petersen, K., Kimmance, S. A., Martinez Martinez, J., Wilson, W. H., Hehemann J.-H., Allen M. J., Sandaa, R.-A. (2014). Dip in the gene pool: metagenomic survey of natural coccolithovirus communities. Virology, 466–467, 129–137. https://doi.org/10.1016/j.virol.2014.05.020

Yawata, Y., Cordero, O. X., Menolascina, F., Hehemann, J.-H., Polz, M. F., & Stocker, R. (2014). Competition-dispersal tradeoff ecologically differentiates recently speciated marine bacterioplankton populations. Proceedings of the National Academy of Sciences of the United States of America, 111(15), 5622–5627. https://doi.org/10.1073/pnas.1318943111

Pluvinage, B., Hehemann, J.-H., & Boraston, A. B. (2013). Substrate recognition and hydrolysis by a family 50 exo-beta-agarase, Aga50D, from the marine bacterium Saccharophagus degradans. The Journal of Biological Chemistry, 288(39), 28078–28088. https://doi.org/10.1074/jbc.M113.491068

Hehemann, J.-H., Correc, G., Thomas, F., Bernard, T., Barbeyron, T., Jam, M., … Czjzek, M. (2012). Biochemical and structural characterization of the complex agarolytic enzyme system from the marine bacterium Zobellia galactanivorans. The Journal of Biological Chemistry, 287(36), 30571–84. https://doi.org/10.1074/jbc.M112.377184

Hehemann, J.-H., Kelly, A. G., Pudlo, N. A., Martens, E. C., & Boraston, A. B. (2012). Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes. Proceedings of the National Academy of Sciences of the United States of America, 109(48), 19786–19791. https://doi.org/10.1073/pnas.1211002109

Hehemann, J.-H., Smyth, L., Yadav, A., Vocadlo, D. J., & Boraston, A. B. (2012). Analysis of keystone enzyme in Agar hydrolysis provides insight into the degradation (of a polysaccharide from) red seaweeds. The Journal of Biological Chemistry, 287(17), 13985–95. https://doi.org/10.1074/jbc.M112.345645

Correc, G., Hehemann, J.-H., Czjzek, M., & Helbert, W. (2011). Structural analysis of the degradation products of porphyran digested by Zobellia galactanivorans β-porphyranase A. Carbohydrate Polymers, 83(1), 277–283. https://doi.org/10.1016/j.carbpol.2010.07.060

Gregg, K. J., Zandberg, W. F., Hehemann, J.-H., Whitworth, G. E., Deng, L., Vocadlo, D. J., & Boraston, A. B. (2011). Analysis of a new family of widely distributed metal-independent alpha-mannosidases provides unique insight into the processing of N-linked glycans. The Journal of Biological Chemistry, 286(17), 15586–15596. https://doi.org/10.1074/jbc.M111.223172

Hehemann, J.-H., Marsters, C., & Boraston, A. B. (2011). Ab initio phasing of a nucleoside hydrolase-related hypothetical protein from Saccharophagus degradans that is associated with carbohydrate metabolism. Proteins, 79(10), 2992–2998. https://doi.org/10.1002/prot.23126

Lammerts van Bueren, A., Ficko-Blean, E., Pluvinage, B., Hehemann, J.-H., Higgins, M. A., Deng, L., … Boraston, A. B. (2011). The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor. Structure (London, England : 1993), 19(5), 640–651. https://doi.org/10.1016/j.str.2011.03.001

Thomas, F., Hehemann, J. H., Rebuffet, E., Czjzek, M., & Michel, G. (2011). Environmental and gut Bacteroidetes: The food connection. Frontiers in Microbiology, 2(MAY), 1–16. https://doi.org/10.3389/fmicb.2011.00093

Hehemann, J. H., Michel, G., Barbeyron, T., & Czjzek, M. (2010). Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 66(Pt 4), 413–417. https://doi.org/10.1107/S174430911000429X

Hehemann, J.-H., Correc, G., Barbeyron, T., Helbert, W., Czjzek, M., & Michel, G. (2010). Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota. Nature, 464(7290), 908–12. https://doi.org/10.1038/nature08937

Ficko-Blean, E., Gregg, K. J., Adams, J. J., Hehemann, J.-H., Czjzek, M., Smith, S. P., & Boraston, A. B. (2009). Portrait of an enzyme, a complete structural analysis of a multimodular {beta}-N-acetylglucosaminidase from Clostridium perfringens. The Journal of Biological Chemistry, 284(15), 9876–9884. https://doi.org/10.1074/jbc.M808954200

Hehemann, J.-H., Redecke, L., Murugaiyan, J., von Bergen, M., Betzel, C., & Saborowski, R. (2008). Autoproteolytic stability of a trypsin from the marine crab Cancer pagurus. Biochemical and Biophysical Research Communications, 370(4), 566–571. https://doi.org/10.1016/j.bbrc.2008.03.128

Hehemann, J.-H., Redecke, L., Perbandt, M., Saborowski, R., & Betzel, C. (2007). Crystallization and preliminary X-ray diffraction studies of trypsin-like proteases from the gastric fluid of the marine crab Cancer pagurus. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 63(Pt 3), 242–245. https://doi.org/10.1107/S1744309107008524