Logo Universitat Bremen
 
Seitenpfad:

Rebecca Aepfler

Doktorand_innen

Gruppe: 

DFG-Emmy-Noether-Gruppe Hydrothermale Geomikrobiologie

Telefon: 

+49 421 218-65965

E-Mail:

Raum: 

Marum Pavillon, 1060

Rebecca Aepfler

PhD project

Shallow-water hydrothermal vent systems:
Lipidomic characterization of microbial communities with focus on heterotrophy

Marine shallow-water hydrothermal vent systems are vastly understudied compared to their deep-sea counterparts; especially the organic chemical composition of the hydrothermal fluids is largely unknown. So far, amino acids (AAs), peptides, and carbohydrates are examples of substrates that are known to provide a source of carbon and energy. Of these, especially dissolved AAs are common sources for both, carbon and nitrogen to microorganisms (Svensson et al., 2004; Rusch et al., 2005) and turn over most rapidly in practically all organisms (Hayes, 2001). Therefore, the overall aim of my PhD is to investigate the microbial degradation of protein biomass in these extreme environments. Respective studies will include the following three approaches:
1. Basic lipid analysis at a new sampling site for studying its microbial diversity and adaptations to extreme conditions
2. Simultaneous Stable Isotope Probing with Deuterium and 13C-DIC for identifying the metabolically active organisms of the detected community and determining its heterotrophic fraction
3. Position-specific labeling using organic carbon substrates for reconstructing the lipid biosyn-thesis on the level of single functional groups

A completely new sampling site for approach 1 in our working group is the Baja California Peninsula (BCP) which extends NW-SE on the western side of the Gulf of California between Mexico and the Pacific Ocean. Approaches 2 and 3 will be conducted with samples originating from Milos (Greece), because the lipidomic approach has been established for this venting site, already. In 2009 and 2012 more precisely, the submarine vents off the Greek island Milos have been studied intensively, providing an ideal natural laboratory to study adaptations of microbial communities to extreme con-ditions (Sollich et al., in prep.).

CV

09/2015 - presentMARUM, University of Bremen
PhD student in the Hydrothermal Geomicrobiology group
PhD topic: Lipidomic characterization of microbial communities with focus on heterotrophy
Supervision: Dr. Bühring (Hydrothermal Geomicrobiology, MARUM)
10/2012 - 07/2015University of Bremen
Master of Marine Geosciences
Master Thesis: Position-specific 13C-labeling in amino acids: A versatile tool to decipher lipid biosynthetic pathways and microbial community response in marine environments
Supervision: Dr. Elvert (Organic Geochemistry, MARUM)
02/2013 - 01/2014University of Waikato, New Zealand
Master of Earth and Ocean Science
10/2009 - 10/2012University of Bremen
Bachelor of Geosciences
Bachelor Thesis: Simultaneous Isolation of Strontium and Lead from Matrix Reference Materials with Sr.Spec
Supervision: Prof. Dr. Kasemann (Isotope Geochemistry, MARUM)

Poster presentation(s)

Rebecca F. Aepfler, Solveig I. Bühring, Marcus Elvert (2015). "Position-specific 13C-labeling in amino acids: A versatile tool to decipher lipid biosynthetic pathways and microbial community response in marine environments". 27th International Meeting on Organic Geochemistry, Prague, Czech Republic, September 2015.

Field trips

21/05 - 31/05/2015Hydrothermal vent sampling campaign at the coast of Kueishantao, Taiwan
Collected DNA, ligand and lipid samples (sediment sampling as well as hydrothermal fluid filtering)
15/09 - 01/10/2014GALIMOS M110 cruise, RV Meteor3 , NW Iberian continental margin, Spain
Planned, controlled and performed CTD profiling and corresponding water sampling, took sediment samples as well as advised water filtering techniques