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Molecular-isotopic Mass-Spectrometry
MAT 253
The ThermoFisher Scientific MAT 253 mass-spectrometer is equipped with a differential pumping system, an universal collector of 8 Faraday cups to measure isotopes of C, O, N, S and 2 dedicated Faraday cups to analyse HD. It uses 10 kV acceleration voltage and has 50 V amplifiers. It is connected to a ConFlo IV referencing interface. Our continuous-flow system is costumized with an oxygen bleed to reduce gas tailing in the ion source.
Inlet systems
Thermal-Conversion/Elemental-Analyser (TC/EA)
The TC/EA is equipped with autosamplers for liquid and solid samples. Samples are pyrolysed in a high-temperature reactor and the resulting H2 and CO gas is transferred via the ConFlo IV into the mass-spectrometer.
Typical uses include isotopic analyses of water (e.g., culture waters, precipitation, seawater) and isotopic determination of solid standards.
Typical precision is ≤ 2 ‰ for HD and ≤ 0.2 ‰ for 18O for liquid analyses and ≤ 3 ‰ for HD and ≤ 0.4 ‰ for 18O for solid analyses.
For questions please contact: 
Gas-chromatograph for lipid analyses
This GC is our work-horse for compound-specific hydrogen isotope analyses. It is equipped with a programmable Triplus autosampler, two injectors (an automated on-column and a PTV injector) allowing most flexible sample injection techniques. After separation in the gas-chromatograph, the eluting compounds are pyrolysed to H2 gas by high-temperature conversion which is transferred into the mass-spectrometer via the ConFlo IV interface. the optional combustion reactor is not used in this system for easier maintenance (see below: ThermoFinnigan MAT 252).
Necessary sample amounts are 200 ng of compound per injection and typical precision is ≤ 3 ‰ for HD.
For questions please contact:
MAT 252
The ThermoFinnigan MAT 252 mass-spectrometer is equipped with an universal collector of 8 Faraday cups for the isotopic analyses of C, N, O, S. It uses 10 kV acceleration voltage and has 10 V amplifiers. The continuous-flow system is not equipped to analyse HD. It is connected to a modified GC/C III interface without reduction furnace.
Inlet systems
Gas-chromatograph for lipid analyses
The gas-chromatograph for lipid analyses is our work-horse for compound-specific 13C analyses and is equipped with a programmable Triplus autosampler and two injectors (automated on-column and large-volume split/splitless injector) allowing highly flexible sample injections. After separation in the gas-chromatograph, the compounds are combusted in a modified combustion reactor filled with Ni-wires and using an oxygen trickle-flow.
Typical precision for compound-specific 13C analyses is ≤ 0.3 ‰.
For questions please contact: